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Plasmid#69352Purposeexpresses human Nox4 in mammalian cellsDepositorInserthNOX4 (NOX4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher
Plasmid#35044DepositorInsertSpyCatcher
UseTagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…PromoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1b
Plasmid#68811PurposeDoxycycline inducible lentiviral vector for FOXM1b expression in mammalian cellsDepositorInsertFOXM1b (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p58sheLL
Plasmid#37324DepositorInsertHPV58L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
UseTagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIH1-puro-control shRNA
Plasmid#26597DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
Plasmid#162382PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2443
Plasmid#72252PurposeHuman expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-A
Plasmid#38132DepositorInsertMITF-A (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH0001
Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 WT puroR
Plasmid#46751PurposeRetroviral vector that expresses wild type Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pyronic/pcDNA3.1(-)
Plasmid#51308PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.DepositorInsertPyronic
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFlag, HA, and Lamp2 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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sgCtr- LentiCRISPRv2
Plasmid#107402PurposeLentiviral expression of Cas9 and a control gRNADepositorInsertcontrol gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mSA-EGFP-TM
Plasmid#39863DepositorInsertmonomeric streptavidin, EGFP, TM helix
UseTagsEGFP, FLAG, and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only