-
Plasmid#43802DepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAP-delta10-cHIS
Plasmid#21716DepositorInsertpolyA polymerase (PAP1 Budding Yeast)
UseTagsHISExpressionBacterialMutation30 amino acids off the c terminusPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804DepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYZ301
Plasmid#102690PurposeS. pombe gRNA entry vector with Cas9 - Sp lys9 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ294
Plasmid#102688PurposeS. pombe gRNA entry vector with Cas9 - KanMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ300
Plasmid#102689PurposeS. pombe gRNA entry vector with Cas9 - Sp his3 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km
Plasmid#46815PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
UseTagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
UseTagsExpressionYeastMutationEnhanced Nucleur Localization SignalPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneUseTagsHIS10-TEV-HAExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pV1093
Plasmid#111428PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3258 pCMV hUBC9 wt HA
Plasmid#14438DepositorInsertUBC9 (UBE2I Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
p109.1
Plasmid#44518DepositorInsertHistone 4
UseTagsGSTExpressionBacterialMutationcontains full length (aa 1-102)PromoterAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p
Plasmid#158141PurposeExpresses Aga1p in yeastDepositorInsertAga1p
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_D17
Plasmid#84611PurposeIntroduces DSB at D17 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3259 pCMV hUBC9 mt HA
Plasmid#14439DepositorInsertUBC9 C93S (UBE2I Human)
UseTagsHAExpressionMammalianMutationCys93Ser mutation (TGC changed to AGC)PromoterCMVAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only