-
Plasmid#52693PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIP5K3
Plasmid#20584DepositorInsertPIP5K3 (PIKFYVE Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-STING-C64S,C65S-FLAG
Plasmid#198186PurposeMammalian expression of FLAG-tagged mouse STING1 C64S, C65SDepositorInsertTMEM173 (Sting1 Mouse)
UseTagsFLAGExpressionMammalianMutationThe cysteine in STING at position 64/65 mutates t…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
UseTagsEPEA and FlagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36
Plasmid#111745PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ36 (polyQ repeat)PromoterCMV and P10Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag MAP2K5
Plasmid#20514DepositorInsertMAP2K5 (MAP2K5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAP-COMP-blac-flag-his
Plasmid#111001PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertaminopeptidase P (APP) (PF14_0517 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
Plasmid#70232PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag MAP3K7
Plasmid#20520DepositorInsertMAP3K7 (MAP3K7 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationQ356HPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Ronin-FLAG-IRES-Neo
Plasmid#28020DepositorInsertRonin (THAP11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-TEX264 LIR4A-FLAG
Plasmid#128259PurposeExpresses TEX264 LIR4A in mammalian cellsDepositorInsertTEX264 LIR4A (TEX264 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationChanged FEEL (aa 273-276) to alaninesPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag MAPK7
Plasmid#20527DepositorInsertMAPK7 (MAPK7 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Tev_ACTA1 (alpha-actin)
Plasmid#188452PurposeExpress in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.DepositorInsertACTA1 (ACTA1 Human)
UseTags6xHis-FLAGExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pbira(1-g78)-sec61b
Plasmid#154455Purposeexpresses flag-pbira(1-g78)-sec61b/biochemical assayDepositorInsertflag-pbira(1-g78)-sec61b (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Linear Tau 0N4R 3X Flag tag
Plasmid#194171PurposeInsert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.DepositorInsertMicrotubule-Associated Protein Tau 0N4R
UseTags3X FlagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAP-GFP
Plasmid#123111PurposeExpresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PDPK1
Plasmid#20565DepositorInsertPDPK1 (PDPK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79
Plasmid#111725PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ79 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag RPS6KB1
Plasmid#20624DepositorInsertRPS6KB1 (RPS6KB1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only