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Plasmid#99979PurposeShuttle yeast Cka1 into Gateway destination vectorDepositorInsertCka1 (CKA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
UseTagsExpressionYeastMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZHY566
Plasmid#50608PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZHY565
Plasmid#50607PurposeTALEN expression vector for yeast, N152,C18 architectureDepositorTypeEmpty backboneUseTALENTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(GiVL-1)
Plasmid#32032DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationH123-N143 replaced by P642-Y645 of GiDicerPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1(1-384)
Plasmid#32031DepositorInsertDCR1(1-384)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(GiVL-2)
Plasmid#32034DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationN195-M215 replaced by P697-D714 of GiDicerPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E147Q/D151N)
Plasmid#32024DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE147Q/D151NPromoterT7Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastMutationPromoterGPDAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIFU002
Plasmid#174299PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, N501YPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU001
Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, E484KPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.A18T
Plasmid#99360PurposeBacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationAlanine 18 to ThreoninePromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.E31K
Plasmid#99361PurposeBacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationGlutamic acid 31 to LysinePromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.110
Plasmid#99363PurposeBacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationAlanine 18 to Threonine and Glutamic acid 31 to L…PromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
Plasmid#58431PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only