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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-ATF7IPi1

Plasmid

#59614

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ATF7IPi2

Plasmid

#59615

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-MED19i1

Plasmid

#59632

Purpose

Expression of shRNA against human MED19

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

MED19 (MED19 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-FKBP-GFP-NDP52

Plasmid

#135296

Purpose

stably express GFP-tagged NDP52 for CID induced mitophagy

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

NDP52 (CALCOCO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116A

Plasmid

#123104

Purpose

Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-p65

Plasmid

#183929

Purpose

Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-p65 (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PVRL2-bio-His

Plasmid

#51824

Purpose

Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PVRL2 (NECTIN2 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VPR

Plasmid

#183928

Purpose

Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VPR (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(+) eGFP p6 K27R

Plasmid

#78583

Purpose

Expresses GFP p6 with K27R mutation in mammalian cells

Depositor

Jeremy Luban

Article

Gurer et al J Virol. 2005 Jan;79(2):910-7.

Insert

eGFP p6

Use

Tags

Expression

Mammalian

Mutation

HIV-1 HXB2 p6 with conservative mutations (G78A, …

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L1

Plasmid

#223571

Purpose

Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116

Plasmid

#123105

Purpose

Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pCMVMagFRET-6-NLS

Plasmid

#50757

Purpose

Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-6 with NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-OCT4

Plasmid

#35391

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Oct4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2012

Availability

Academic Institutions and Nonprofits only
pCB6-ZONAB-B

Plasmid

#136642

Purpose

Expression of ZONAB isoform B in mammalian cells

Depositor

Karl Matter, Maria Balda

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform B (YBX3 Canine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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