Addgene

Showing: 4181 - 4200 of 8561 results

Results per page:

Sort results by:

pRB38

Plasmid

#72864

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF290

Plasmid

#72859

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pexA2_ring

Plasmid

#89672

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

ring

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pexA2_2hb_ruleB

Plasmid

#89670

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the two-helix-bundle shown in figure 2E,F,G of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

2hb_ruleB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLAP

Plasmid

#200786

Purpose

Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Type

Empty backbone

Use

Tags

CLIPf and SNAPf-6xHis-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
in-frame ampicillin PKAKTR backbone

Plasmid

#177197

Purpose

PKAKTR mutagenesis template with in-frame ampicillin site

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-RFP_mmu-miR-292b-3p_sponge

Plasmid

#132623

Purpose

Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

Depositor

Constance Ciaudo

Insert

stop codon, mmu-miR-292b-3p sponge sequence and polyA signal

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-Phi6-P2

Plasmid

#182535

Purpose

Template for production of Phi6 P2 RNA

Depositor

Peter Sarin

Article

Gregorova et al MethodsX. 2022 May 23;9:101737. doi: 10.

Insert

P2 pol

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZV_21_400

Plasmid

#170477

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Voros et al Protein Sci. 2017 Jul;26(7):1427-1438. d

Insert

T7A1, O2, O1, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pexK4_cross

Plasmid

#89677

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the cross shown in figure 3G in the associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

cross

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pexK4_drigalski

Plasmid

#89676

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the Drigalski spatula shown in figure 3E of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

drigalski

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pexK4_square

Plasmid

#89674

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the square shown in figure 3F of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

square

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pexK4_wm_ruleB

Plasmid

#89675

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the three-armed star shown in figure 3D of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

wm_ruleB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Use

Tags

C-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM1303

Plasmid

#40243

Depositor

Susan Golden

Article

Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

Insert

Sp/Sm Resistance, neutral site I integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge