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Showing: 401 - 420 of 457 results

  1. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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  10. pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

    Plasmid
    #179902
    Purpose
    Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    antiGFPnanobody-mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179902-plasmid-map-sequence-id-357194

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  15. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  18. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

    Plasmid
    #204151
    Purpose
    Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Insert
    SARS-CoV-2 Delta variant spike
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    TRE3G
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204151-plasmid-map-sequence-id-404782

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Showing: 401 - 420 of 457 results