-
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY1243-Fluorescent RADARS
Plasmid#182540PurposeExpress Fluorescent RADARS in bicistronic vector with mCherry as control geneDepositorInsertmNeon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRCH
Plasmid#136926PurposeExpresses Cas9-NRCH in mammalian cellsDepositorInsertCas9-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max
Plasmid#138155PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1461_SERPINA1_CCA32_5avidity Cre
Plasmid#193193PurposeExpress Cre conditional upon RNA transcriptDepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-H2AZ-halo
Plasmid#197907Purposesingle-particle tracking for H2AZDepositorInsertH2az2 (H2az2 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
EE-BE4max
Plasmid#138158PurposeC-to-T base editorDepositorInsertrAPOBEC1(EE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 R126E + R132E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNb4OMT-A53M
Plasmid#216232PurposeExpresses a mutant Norbelladine 4'-O-MethyltransferaseDepositorInsertNb4OMT-A53M
UseSynthetic BiologyTagsExpressionMutationA53MPromoterP150Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRTH
Plasmid#136925PurposeExpresses Cas9-NRTH in mammalian cellsDepositorInsertCas9-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertNDM-1 (blaNDM-1 Klebsiella pneumoniae)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
YE2-BE4max
Plasmid#138156PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE2)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R132E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-mF(3230)A3
Plasmid#168016PurposeExpresses MBP-F(3230)A3-6xHis in bacterial cellsDepositorInsertMBP-F(3230)A3-6xHis
UseTagsHisx6 and maltose binding proteinExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-CP1028
Plasmid#138160PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgLenti
Plasmid#105996Purposesingle sgRNA expression vectorDepositorTypeEmpty backboneUseCRISPR and Lentiviral; S.pyogenes sgrna expressio…TagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AV44_pCAG.Cas9-D10A.gRNA.S1
Plasmid#199258PurposeExpression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNADepositorInsertsSpCas9-D10A nickase
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only