pSL7 SMR1791
(Plasmid #28030)

• Depositing Lab: Susan Rosenberg
• Publication: Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 4361
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MutS, MutL
• Alt name: methyl-directed mismatch repair protein
• Species: E. coli
• Entrez Gene: mutL (a.k.a. b4170, ECK4166)
• Entrez Gene: mutS (a.k.a. b2733, ECK2728, ant, fdv, plm)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL)