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pY71-tet.I.5

Plasmid

#133527

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.3

Plasmid

#133525

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.2

Plasmid

#133524

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.4

Plasmid

#133526

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.1

Plasmid

#133523

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT100-neo

Plasmid

#123137

Purpose

Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Neomycin, poly-A terminator

Use

Synthetic Biology; Golden gate donor vector

Tags

Expression

Mutation

Promoter

none

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-shMcu-1

Plasmid

#181868

Purpose

Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

hrGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-shMcu-2

Plasmid

#181867

Purpose

Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

hrGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico p53

Plasmid

#12089

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico Dnmt1

Plasmid

#12165

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

Dnmt1 shRNA (Dnmt1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pSicoR p53

Plasmid

#12090

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR Dnmt1

Plasmid

#12166

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

Dnmt1 shRNA (Dnmt1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pLenti 4.1 Ex miR200b-200a-429

Plasmid

#35533

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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mCh-2xFKBP (no insert) (pBS1139)

Plasmid

#185322

Purpose

The empty vector used for the FKBP experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only
AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE3_D125I_mCh-2xFKBP (pBS1157)

Plasmid

#185330

Purpose

For the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I

Use

Tags

Expression

Mammalian

Mutation

D125I

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE3_mCh-2xFKBP (pBS1156)

Plasmid

#185329

Purpose

For the mammalian expression of the human protein ApoE3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)

Plasmid

#185320

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge