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pLKO.1 RagD shRNA #2
Plasmid#30322DepositorInsertRagD shRNA (RRAGD Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 K530A
Plasmid#51716Purposeplasmid expresses FLAG/HA-tagged MOV10 K530ADepositorInsertMOV10 K530A (MOV10 Human)
UseTagsFLAG/HAExpressionMammalianMutationK530APromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA MOV10 D645N
Plasmid#51717Purposeplasmid expresses FLAG/HA-tagged MOV10 D645NDepositorInsertMOV10 D645N (MOV10 Human)
UseTagsFLAG/HAExpressionMammalianMutationD645NPromoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-NGLY1-Puro
Plasmid#181916PurposeLentiviral plasmid that directs the expression of NGLY1 in mammalian cells.DepositorInsertN-glycanase 1 (NGLY1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-AKT1-V5-miniTurbo_IDG-K
Plasmid#189889PurposeGateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-AKT1_IDG-K
Plasmid#189890PurposeGateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPR83-Tango
Plasmid#66376PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR83 (GPR83 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-H2B-C-10
Plasmid#57509PurposeLocalization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertH2B (H2BC11 Human)
UseTagsmEos4bExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSH1/Mf-del-Akt-E
Plasmid#15288DepositorInsertdelPH-Akt (Akt1 Mouse)
UseTagsHA epitope and Myristoylation-targeting domain Fy…ExpressionMammalianMutationTruncated PH (residues 1-99)PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-192
Plasmid#26311DepositorInserthsa-mir-192 (MIR192 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (71-181)
Plasmid#86753PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 71-181PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (396-492)
Plasmid#86755PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 396-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (181-413)
Plasmid#86754PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 181-413PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (1-181)
Plasmid#86752PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 1-181PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (201-492)
Plasmid#86751PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 201-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
UseTagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (1-200)
Plasmid#86750PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertAtg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 1-200PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
