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Plasmid#193134PurposeA2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).DepositorInsertGB_SynP (A2) G1b.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB2891
Plasmid#193128PurposeA2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.DepositorInsertGB_SynP (A2) G1abc.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB2885
Plasmid#193123PurposeA2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.DepositorInsertGB_SynP (A2) G1ab.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB2879
Plasmid#193116PurposeA2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.DepositorInsertGB_SynP (A2) G1aG2b.5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB3278
Plasmid#193132PurposeA2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.DepositorInsertGB_SynP (A2) G1abc.5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB3273
Plasmid#193135PurposeA2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).DepositorInsertGB_SynP (A2) G1e.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-Venus
Plasmid#186372PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP4G-REL2N91-ccdB
Plasmid#188444PurposeZea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDESTDepositorInsertREL2N91/ccdB
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL6730
Plasmid#139155PurposePapFAB45 - TARGET3 - MevT - MBISDepositorInsertMevT-MBIS
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL6731
Plasmid#139156PurposePstabilized - TARGET8 - MevT - MBISDepositorInsertMevT-MBIS
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL6721
Plasmid#139152PurposePDNsp1 - TARGET8 - sfGFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL6722
Plasmid#139153PurposePstabilized - TARGET8 - sfGFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL6729
Plasmid#139154PurposePgadE - TARGET3 - MevT - MBISDepositorInsertMevT-MBIS
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoLuc
Plasmid#135284PurposeGBPart - RoLuc Luciferase CDSDepositorInsertR. ohbai Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C'4800EC
Plasmid#176875PurposeEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 ToolkitDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSM160
Plasmid#105684PurposePlasmid containing an RFP dropout used to construct randomized PAM libraryDepositorInsertRFP Golden Gate dropout
UseTagsExpressionBacterialMutationPromoterBba_R0040 TetR PromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only