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Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (WT)
Plasmid#200978PurposeMammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding DomainPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-11
Plasmid#72349PurposeMammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-hRnf185
Plasmid#175127PurposeLentiviral expression of V5-tagged human RNF185DepositorInsertRNF185 (RNF185 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR-TNF
Plasmid#153069PurposeHuman TNF 3'UTR region cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of TNF gene (TNF Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-dL5-2XG4S-mCer3
Plasmid#73207PurposeExpresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)DepositorInsertKozATG-dL5-2XG4S-mCer3
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
Plasmid#153071PurposeLentiviral vector expressing human ZC3H12D mutated (D95N)DepositorInsertMutated ZC3H12D (D95N) (ZC3H12D Human)
UseLentiviralTagsExpressionMammalianMutationChanged D95 to N, known to abrogate the RNase enz…PromoterEF1αAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-BHLHE40-IRES-ZsGreen1
Plasmid#153061PurposeLentiviral vector expressing human BHLHE40DepositorInsertBHLHE40 (BHLHE40 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPar2
Plasmid#53228Purposemammalian expression of human Par2DepositorInsertPar2 (F2RL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailable sinceDec. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-ProDOL
Plasmid#206866PurposeExpression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTagDepositorArticleInsertProDOL
UseRetroviralTagsHaloTag, His-Tag, SNAP-tag, and eGFPExpressionMammalianMutationContains Lyn amino acids 1-16PromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK p2A mCherry
Plasmid#117693PurposeCAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs peptide
UseCre/LoxTagsHA / FLAGExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB2
Plasmid#200982PurposeMammalian expression plasmid for myc-tagged EphB2DepositorInsertEphB2 (EPHB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
DPYSL2-pLJC2
Plasmid#189866PurposeExpresses human DPYSL2 (CRMP2) in mammalian cellsDepositorInsertDPYSL2 (DPYSL2 Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-AnillinFL
Plasmid#187271PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationPromoterU6, CMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-Anillin shRNA
Plasmid#187270PurposeLentiviral expression of shRNA targeting AnillinDepositorInsertshRNA for Anillin (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-PB-SRT-tdTomato
Plasmid#154889PurposepiggyBac self-reporting transposon with tdTomato marker in AAV backboneDepositorInsertpiggyBac SRT
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only