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Plasmid#208025PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
UseTagsHis6 TagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-Electra2
Plasmid#184930PurposeTubulin labeling with blue fluorescent protein Electra2DepositorInsertTUBA1B-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AP2u2-mCherry
Plasmid#27672DepositorInsertAp2m1 (Ap2m1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-7
Plasmid#199212PurposeLanding Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection markerDepositorInsertsaminoglycoside phosphotransferase,
enhanced Green Fluorescence Protein
Red Fluorescence Protein
UseTagsExpressionMammalianMutationPromoterCMV and thymidine kinase promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mitoGFP-DIO
Plasmid#174112PurposeCre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoterDepositorInsertmitoGFP (COX8A Human)
UseAAVTagsCox8 targeting sequence and GFPExpressionMutationPromoterEf1aAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLX304_zeo_mmIrf1
Plasmid#160098PurposeExpresses murine Irf1 in mammalian cellsDepositorInsertIrf1 (Irf1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP‐RNASEH2A
Plasmid#108700PurposeFor expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cellsDepositorInsertRNASEH2A (RNASEH2A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mDlx-SynTagMA_post-2A-mCerulean
Plasmid#130899PurposeSynaptic Tag for Mapping Activity in GABAergic neuronsDepositorInsertSynTagMA
UseAAVTagsmCeruleanExpressionMammalianMutationPromotermDlxAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
Plasmid#21850DepositorInsertATF4 (Atf4 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationATF4 5' UTR and AUG fused to Luciferase driv…PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CenpA-N-22
Plasmid#62742PurposeLocalization: Nucleus, N1 Cloning Vector, Excitation:587, Emission:610DepositorInsertCenpA (CENPA Human)
UseTagsmCherryExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCM157
Plasmid#45863DepositorInsertcre recombinase
UseCre/LoxTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CD
Plasmid#101921PurposeThe dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).DepositorInsertdCas9-TET1CD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ARAF
Plasmid#116713PurposeLentiviral expression of ARAFDepositorInsertARAF (ARAF Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKW0770
Plasmid#119023PurposeE.coli expression plasmid for PmSPS1DepositorInsertPmSPS1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
px458_Conc2
Plasmid#134449PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pProEx-HTa-SOD1 WT
Plasmid#191849Purposeexpress wild-type SOD1 in bacterial cellsDepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor
Plasmid#26633DepositorInsertraptor (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 alpha
Plasmid#20351DepositorInsertpDCNA3 Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJAT17
Plasmid#204298PurposeFor introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.DepositorInsert3XP3-EYFP
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYS1Ptac
Plasmid#85545PurposeConstitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoterDepositorInsertsCpf1
recT
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromotertac and unknownAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_hsTRPA1_myc_dTom
Plasmid#183179Purposemyc tagged hsTRPA1 used for cell transfectionDepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-YFP
Plasmid#166897Purposemammalian expression of Sar1b tagged with YFPDepositorInsertSar1b
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 - T7 pro - IRES - EGFP
Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterPvegAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-GBDpol (exo)
Plasmid#165572PurposeRobust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEBDepositorInsertPyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
ALKBH5H204A-dCas9
Plasmid#134784PurposeExpression fusion protein ALKBH5H204A-dCas9 in Mammalian cellsDepositorInsertALKBH5 and inactive dCas9
UseTagsExpressionMammalianMutationH204APromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJKW0957
Plasmid#119026PurposeE.coli expression plasmid for PmKOMT1DepositorInsertPmKOMT1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16
Plasmid#158742PurposeExpresses influenza A virus tetrameric neuraminidase fused to a sfGFPDepositorInsertNeuraminidase A/NL/354/16 H3N2
UseTagsTwinStrep_sfGFP_tetrabrachionExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-SEP-mRuby3
Plasmid#200939Purposeendolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-APEX2
Plasmid#154936PurposeNLS-V5-MCP-APEX2DepositorInsertNLS-V5-MCP-APEX2
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO plasmid
Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLJM1 RagA
Plasmid#73031PurposeExpresses human RagA from a CMV promoter for stable expression in mammalian cellsDepositorInsertrragA (RRAGA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 DN
Plasmid#12662DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-egfp-U3-Osp.gRNA
Plasmid#166034PurposeEmpty backbone for expression of gRNA-expressing integration deficient lentiviral particles.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Blast (w818-1)
Plasmid#27568DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWB554
Plasmid#74884PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (PGK-Puro)
Plasmid#63214PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection markerDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis
Plasmid#157142PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPILRA (PILRA Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only