-
Plasmid#136709PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr371DepositorInsertOlfr371
UseIn situ probeTagsExpressionMutationISH Probe insertPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Olfr398
Plasmid#136711PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr821DepositorInsertOlfr821
UseIn situ probeTagsExpressionMutationISH Probe insertPromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pexK4_4hb_straight
Plasmid#89678PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the straight four-helix-bundle shown in figure 4A of associated article.DepositorInsert4hb_straight
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_4hb_bent
Plasmid#89679PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent four-helix-bundle shown in figure 4E of associated article.DepositorInsert4hb_bent
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_bow_360
Plasmid#89673PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3B of associated article.DepositorInsertbow_360
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexA2_bow_180
Plasmid#89671PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3A of associated article.DepositorInsertbow_180
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKLP-tetA
Plasmid#71325PurposetetA landing pad template plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRKH2
Plasmid#71312PurposeTheta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB4581
Plasmid#171124PurposeA kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.DepositorInsert3_sAID
UseOtherTagsExpressionMutationnon applicablePromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-SpyCatcher-mi3
Plasmid#113043PurposeExpresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertHis6-SpyCatcher-mi3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorInsertsCopGFP
mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-116:PCNA-mEGFP
Plasmid#170818PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human PCNADepositorInsertPCNA Homology Arms with mEGFP-linker (PCNA Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits