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pFX-Lifeact-Sirius

Plasmid

#174475

Purpose

Express Sirius with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Use

Tags

Cmyc and HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pVR2-m6

Plasmid

#84725

Purpose

pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer

Depositor

Nils Walter

Article

Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

Inserts

E. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene

Use

Tags

Expression

Bacterial

Mutation

insertion of 2 Us between positions 39 and 40 of …

Promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-ADAM7i2

Plasmid

#59603

Purpose

Expression of shRNA against human ADAM7

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM7 (ADAM7 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i2

Plasmid

#59597

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i1

Plasmid

#59596

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pαH-S-RRAR-OMICRON.BA.5

Plasmid

#213071

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-RRAR-OMICRON.BA.5 (S )

Use

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685, furin site inta…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Sabrina Spencer, Tobias Meyer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4

Plasmid

#157796

Purpose

Expression of human linker histone H1.4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4 (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro c-met WT

Plasmid

#17493

Depositor

Joan Brugge

Article

Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

Insert

c-Met (Met Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAV5B+longBRD4

Plasmid

#157792

Purpose

Expression of long isoform of BRD4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

long BRD4 (BRD4 Human)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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GBSGFP insulator

Plasmid

#121975

Purpose

Gli activity reporter, mouse transgenesis

Depositor

James Briscoe

Article

Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

Insert

GBS(long)-GFP-insulator (Gnb5 Mouse)

Use

Mouse transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only
NG0750 pCI-TPI-PTC48-4H

Plasmid

#65803

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hML23Cm6

Plasmid

#109368

Purpose

Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5RS-HERCD533

Plasmid

#65224

Purpose

Expression of dominant negative EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

Insert

HERCD533 (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

deleted AA 654-1186

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pPRIME-CMV-GFP-shDyn2

Plasmid

#132712

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(2) (Pdyn Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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PHR-GFP

Plasmid

#183926

Purpose

Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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