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Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
UseTagsHygromycin phosphotransferaseExpressionMammalianMutationPromoternoneAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-TJP1
Plasmid#227299PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TJP1 (TJP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4807
Plasmid#215273PurposeModule for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19.DepositorInsertPNOS:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4808
Plasmid#215274PurposeModule for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19.DepositorInsertP35S:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4832
Plasmid#215276PurposeModule for the constitutive expression of HISPS, H3H, EGFP and p23.DepositorInsertHISPS-H3H-EGFP-p23
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4485
Plasmid#215258PurposeModule for the constitutive expression of the N. nambi CPH, LUZ, H3H genes, EGFP and p19 fused to a SF in alpha2.DepositorInsertSF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4468
Plasmid#215257PurposeModule for the constitutive expression of the N. nambi LUZ, H3H genes, EGFP and p19 fused to a SF in omega2.DepositorInsertSF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4665
Plasmid#215270PurposeModule for the expression of nptII and the N. nambi LUZ and CPH genes flanked by insulators on the sides of the construction.DepositorInsertMAR10-SF-nptII-LUZ-CPH-MAR10-SF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV107
Plasmid#215337PurposeExpresses sfGFP under induction with the DHBA Marionette Cassette (PcaUAM + P3B5B-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterP3B5BAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Cas9-2A-GFP
Plasmid#170159PurposeExpress Cas9 and GFP upon doxycycline treatmentDepositorInsertCas9
UseSynthetic Biology; PiggybacTagsGFPExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4193
Plasmid#215251PurposeTU for the expression of the CIB1 gene fused to the VPR activation domain under the P35S promoter and TNOS terminator in alpha1DepositorInsertP35S:CIB1:VPR:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.B-GS3
Plasmid#204760PurposeExpression of Cas9 and human H2A.BDepositorInsertH2AB1 (H2AFB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH3.2-GS3
Plasmid#204763PurposeExpression of Cas9 and human H3.2DepositorInsertH3.2 (HIST2H3PS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.L-GS3
Plasmid#204756PurposeExpression of Cas9 and human H2A.LDepositorInsertH2AL3 (H2AC6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ipYUC2Cas-LT1
Plasmid#210559PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.DepositorInsertpMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.1
Plasmid#208306PurposeExpresses ABE-RA4.1 in mammalian cellsDepositorInsertTadA-RA4.1-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.2
Plasmid#208307PurposeExpresses ABE-RA4.2 in mammalian cellsDepositorInsertTadA-RA4.2-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.3
Plasmid#208308PurposeExpresses ABE-RA4.3 in mammalian cellsDepositorInsertTadA-RA4.3-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only