Addgene

We narrowed to 16,226 results for: multi

Showing: 4121 - 4140 of 16226 results

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Tags

Expression

Mutation

NLS fusion

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-FUS-G156E

Plasmid

#181716

Purpose

Galactose inducible expression of FUS-G156E

Depositor

Alejandro Chavez

Insert

FUS (FUS Human)

Use

Tags

Expression

Yeast

Mutation

G156E

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-TKBsd-L2

Plasmid

#141009

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKBsd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pNIR_PKAbiosensor

Plasmid

#127439

Purpose

Mammalian expression of the NIR biosensor for detecting PKA activity

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

NIR PKA biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pNIR_JNKbiosensor

Plasmid

#127440

Purpose

Mammalian expression of the NIR biosensor for detecting JNK activity

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

NIR JNK biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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