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Plasmid#200786PurposeCloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.DepositorTypeEmpty backboneUseTagsCLIPf and SNAPf-6xHis-FLAGExpressionMammalianMutationPromoterAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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in-frame ampicillin PKAKTR backbone
Plasmid#177197PurposePKAKTR mutagenesis template with in-frame ampicillin siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-RFP_mmu-miR-292b-3p_sponge
Plasmid#132623PurposeExpresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA spongesDepositorInsertstop codon, mmu-miR-292b-3p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-Phi6-P2
Plasmid#182535PurposeTemplate for production of Phi6 P2 RNADepositorInsertP2 pol
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZV_21_400
Plasmid#170477PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O2, O1, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pexK4_cross
Plasmid#89677PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the cross shown in figure 3G in the associated article.DepositorInsertcross
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_drigalski
Plasmid#89676PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the Drigalski spatula shown in figure 3E of associated article.DepositorInsertdrigalski
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_square
Plasmid#89674PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the square shown in figure 3F of associated article.DepositorInsertsquare
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_wm_ruleB
Plasmid#89675PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the three-armed star shown in figure 3D of associated article.DepositorInsertwm_ruleB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9663 - ACTB GFP-P2A CLIP Donor
Plasmid#202688PurposeCLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.DepositorInsertCopGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
UseTagsC-tagExpressionBacterialMutationPromoterT7Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM1303
Plasmid#40243DepositorInsertSp/Sm Resistance, neutral site I integration platform for Synechococcus
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AC
Plasmid#108686PurposePlasmid for assembly of donor DNA templates consisting of two expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-A114-N40
Plasmid#50561PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneUseTagsCRUExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_NT
Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorInsertsgRNA pyk (NT)
UseCRISPRTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_T
Plasmid#74066PurposepAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecRDepositorInsertsgRNA pgi (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only