-
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
CeNL-Actin_pcDNA3
Plasmid#89512PurposeCyan color variant of bright luminescent protein, fused to actinDepositorInsertCeNL
UseTagsActinExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubi:Zebrabow
Plasmid#118219PurposeFor ubiquitous expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-RFP
Plasmid#1814DepositorInsertGag (gag MuLV)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-G-Cdc42
Plasmid#180577PurposeddGFP sensor of Cdc42DepositorInsertddGFP sensor of Cdc42
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA(tyr)
Plasmid#64250Purposeexpresses sgRNA(tyr) under U6a promoterDepositorInsertU6a:sgRNA (tyr)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9C
Plasmid#80931PurposeExpresses Cas9C in mammalian cells.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
apt-CBE-3'end
Plasmid#219939PurposeMammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP markerDepositorInsertMCP_32-aa_evo-rA1_2xUGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
apt-ABE8e
Plasmid#219937PurposeMammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP markerDepositorInsertCom_93-aa_TadA8e
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag9
Plasmid#175543PurposeExpression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185K/C280S in pET-15b
Plasmid#61669PurposeHIV-1 integrase F185K/C280S in pET-15bDepositorInsertHIV-1 integrase F185K/C280S
UseSynthetic BiologyTagsHisExpressionMutationPromoterT7 promotorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-R-Hras
Plasmid#180574PurposeddRFP sensor of HrasDepositorInsertddRFP sensor of Hras
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP
Plasmid#162229PurposeLentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C
Plasmid#184410Purposebacterial expression of TTRDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85CPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-G-Kras
Plasmid#180571PurposeddGFP sensor of KrasDepositorInsertddGFP sensor of Kras
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only