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Showing: 4081 - 4100 of 22178 results

  2. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pHB4

    Plasmid
    #53462
    Purpose
    This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.
    Depositor
    Elizabeth Conibear
    Article
    Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11
    Insert
    yeGFP-Snc1-Suc2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    53462-plasmid-map-sequence-id-83347

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  4. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Nils Walter
    Article
    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

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  17. pB2Hopt

    Plasmid
    #163830
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163830-plasmid-map-sequence-id-337586

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  18. KBB96

    Plasmid
    #185113
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Last FXFG domain of Nup1
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185113-plasmid-map-sequence-id-368072

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  19. pB2H6

    Plasmid
    #163835
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163835-plasmid-map-sequence-id-337603

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Showing: 4081 - 4100 of 22178 results