-
Plasmid#64107PurposeExpression of Sp dCas9-3XGFP in mammalian cellsDepositorInsertSp dCas9
UseLentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP
Plasmid#73030PurposeExpress copGFPDepositorInsertcopGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_HaloTag7_T2A_EGFP
Plasmid#169325PurposeExpression of HaloTag7 in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-Sec23B
Plasmid#66598PurposeExpresses Sec23B as a fusion with mGFPDepositorInsertSec23B (SEC23B Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-EGFP
Plasmid#61462PurposeAAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpADepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa
Plasmid#127389PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alphaDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pg-HIF-1alpha-EGFP
Plasmid#87204PurposeC-terminally EGFP-tagged human HIF-1aDepositorInsertHIF-1a (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationL40F, M88V, Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -1000 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-GFP
Plasmid#66080PurposeThis G protein alpha-q construct contains internal insertions of GFP and the EE epitopeDepositorInsertG protein alpha-q-EE-YFP (Gnaq Mouse)
UseTagsGFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tcf/Lef:H2B-GFP
Plasmid#32610DepositorInsertTcf/Lef:H2B-GFP
UseTagsExpressionMutationPromoterhsp68Available sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP-VPR
Plasmid#181907PurposeFluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-RtaDepositorInsertdCas9-VPR
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK562 (pDS-GFP)
Plasmid#183129PurposeControl plasmid that expresses off-target dsRNADepositorInsertGFPmut3 (no RBS)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-wt
Plasmid#12980DepositorInsertRac1 (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-EGFP
Plasmid#14203DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-palGFP
Plasmid#190232PurposeAAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cellsDepositorInsertEGFP
UseAAVTagspalmitoylation signal sequenceExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma2-GFP2
Plasmid#166774PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma2-GFP2 (GNG2 Human, Synthetic)
UseTagsExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorInsertbeta actin (ACTB Human)
UseTagsPAGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only