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pDEST-CMV 3xFLAG-GABARAPL2-GFP

Plasmid

#123113

Purpose

Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-RINT1

Plasmid

#40124

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

RINT1 (RINT1 Human)

Use

Lentiviral

Tags

V5 epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 880-1038

Plasmid

#53350

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 880-1038

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 224-454

Plasmid

#53347

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 224-454

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 1039-1176

Plasmid

#53351

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1039-1176

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
Lyn-iTrkA-mCh-CRY2

Plasmid

#106169

Purpose

mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

Lyn iTrkA mCherry CRY2PHR (CRY2 Mustard Weed, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Enhancer-ZC3H12D_PromoterWT

Plasmid

#153066

Purpose

ZC3H12D promoter region cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region (ZC3H12D Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pET21a(+) LdtMt2 (131-408)

Plasmid

#59337

Purpose

Expresses LdtMt2 for L,D-transpeptidation

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

LdtMt2 (131-408)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
ABE8e(TadA-8e K20A R21A)

Plasmid

#138496

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e K20A R21A)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

TadA-8e K20A R21A

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2 V-1

Plasmid

#84804

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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