Showing: 4081 - 4100 of 18075 results
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Plasmid#53178PurposeExpresses mouse CMYC T58A from puromycin resistance retroviral vectorDepositorInsertCMYC (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationT58APromoterAvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman 7SKAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 VP64-T2A-GFP
Plasmid#53192PurposeCo-expresses human optimized S. pyogenes dCas9-VP64 and GFPDepositorInserthumanized dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1
Plasmid#53195PurposeExpresses HA tagged human MEK1 from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-ERK2 GOF
Plasmid#53203PurposeExpresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vectorDepositorInsertERK2 (MAPK1 Human)
UseRetroviralTagsHAExpressionMammalianMutationR67S/D321NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCLucIPZ
Plasmid#53222PurposeConstitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailabilityAcademic Institutions and Nonprofits only -
pBJ FLAG-hPar1
Plasmid#53226Purposemammalian expression of human Par1 with N-terminal FLAG tagDepositorInsertPar1 (F2R Human)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-39 (native signal peptide)PromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPar2
Plasmid#53228Purposemammalian expression of human Par2DepositorInsertPar2 (F2RL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SuperNova/pRSETB
Plasmid#53234Purposemonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertmonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a/Cas9-Cys
Plasmid#53261PurposeExpresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal CysteineDepositorInsertCas9-Cys
UseTags6X His tag, HA epitope, and NLSExpressionBacterialMutationAdditional Cysteine is added to the C-terminal re…PromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailabilityAcademic Institutions and Nonprofits only
Showing: 4081 - 4100 of 18075 results