Addgene

We narrowed to 8,438 results for: plat

Showing: 4061 - 4080 of 8438 results

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pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-69: NPM1-mTagRFP-T

Plasmid

#124608

Purpose

Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

Depositor

Allen Institute for Cell Science

Insert

NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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SOX9-T2A-NLS-tdTomato-PGK-Puro

Plasmid

#89993

Purpose

Donor template for generation of SOX9-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-77:TERF2-mEGFP

Plasmid

#168798

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human TERF2.

Depositor

Allen Institute for Cell Science

Insert

TERF2 (TERF2 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

April 14, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Use

Tags

Expression

Mutation

Promoter

Available since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ8079 - IL2RG GFP-P2A CLIP Donor

Plasmid

#202690

Purpose

CLIP donor template for integrating GFP into IL2RG locus.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEP-EGFPin

Plasmid

#60961

Purpose

PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

eGFP-Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB37

Plasmid

#72869

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTLR-donor

Plasmid

#118999

Purpose

Repair template for the TLR reporter

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pRB38

Plasmid

#72864

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pKF290

Plasmid

#72859

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pexA2_ring

Plasmid

#89672

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

ring

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pexA2_2hb_ruleB

Plasmid

#89670

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the two-helix-bundle shown in figure 2E,F,G of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

2hb_ruleB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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