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Plasmid#222890PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2130-FKBP-GPA-114-20-CyOFP1
Plasmid#222889PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G_LF(C199S)
Plasmid#216112PurposeExpresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.DepositorInsertoROS-G_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-SUN1ΔN
Plasmid#213508PurposeExpresses human eGFP-SUNΔN in mammalian cellsDepositorInserteGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP-SUN1ΔN
Plasmid#213516PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cellsDepositorInsertKU70-eGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-oROS-HT
Plasmid#216416PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.DepositorInsertpDisplay-oROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2-GFP (A1134-1135)
Plasmid#204203PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationGly to Ala 1134, 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (STOP)
Plasmid#204204PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationNonsense mutations after Gly1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (A1134-1135)
Plasmid#204201PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationGly to Ala 1134 to 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-FR-2Strep
Plasmid#210312PurposeExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SEC61B-2Strep
Plasmid#210375PurposeExpresses SEC61B fused with 2Strep in mammalian cellsDepositorInsertSEC61B (SEC61B Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TMEM106B-2Strep
Plasmid#210360PurposeExpresses TMEM106B fused with 2Strep in mammalian cellsDepositorInsertTMEM106B (TMEM106B Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 D-4
Plasmid#184016PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 651 to 665.DepositorInsertProm1 SV8(-Ex19a) D-4 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 Del EC2
Plasmid#184025PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.DepositorInsertProm1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 D-6
Plasmid#184018PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 631 to 645.DepositorInsertProm1 SV8(-Ex19a) D-6 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 D+3
Plasmid#184022PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 721 to 735.DepositorInsertProm1 SV8(-Ex19a) D+3 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only