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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP5

Plasmid

#220071

Purpose

overexpresses ORP5 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-jRCaMP1b

Plasmid

#127873

Purpose

Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Red-shifted mitochondrial matrix Ca2+ indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(H840A)-T2A-EGFP

Plasmid

#221231

Purpose

Express nCas9 nickase (H840A) with GFP reporter

Depositor

Fei Chen

Article

Chen et al bioRxiv 2024.02.01.577593

Insert

SpCas9(H840A)-T2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

H840A

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM22082_sfGFP1-10

Plasmid

#153515

Purpose

E.coli expression plasmid for the production of GFP1-10

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

sfGFP1-10

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Article

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAXY0001

Plasmid

#179834

Purpose

binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2_166

Plasmid

#199098

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_167

Plasmid

#199099

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_170

Plasmid

#199102

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_176

Plasmid

#199108

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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cyto-Ruby3-iATPSnFR1.0

Plasmid

#102551

Purpose

expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

Ruby3-iATPSnFR1.0

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-LOV2

Plasmid

#81009

Purpose

Expression of LOV2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

NTOM20

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV, p10

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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