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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNS3_Ptrc::lasR_Plas::mNG

Plasmid

#189573

Purpose

Fluorescent reporter for Las activation by 3OC12-HSL

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Inserts

Transcriptional activator lasR
Monomeric Neon Green

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

las and trc

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC Renilla Luc SRE

Plasmid

#191657

Purpose

Expressed Renilla Luciferase with SOX resistance element

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

Renilla Luciferase SRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pM-EBFP2

Plasmid

#228614

Purpose

Mammalian expression of EBFP2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mT-Sapphire

Plasmid

#228616

Purpose

Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mT-Sapphire

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mAmetrine

Plasmid

#228617

Purpose

Mammalian expression of mAmetrine and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mAmetrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mCerulean3

Plasmid

#228618

Purpose

Mammalian expression of mCerulean3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mCerulean3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-EGFP

Plasmid

#228622

Purpose

Mammalian expression of EGFP and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mClover3

Plasmid

#228624

Purpose

Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mVenus

Plasmid

#228625

Purpose

Mammalian expression of mVenus and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-miRFP670

Plasmid

#228631

Purpose

Mammalian expression of miRFP670 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

ugpB

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3481 MTK3-mCherry

Plasmid

#226672

Purpose

MTK3 part containing the coding sequence of mCherry.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Removed BbsI sites

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2670 MTK3-PEmax

Plasmid

#226663

Purpose

MTK3 part containing the coding sequence of PEmax

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

PEmax

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-HA

Plasmid

#222711

Purpose

Expression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5c-cdl

Plasmid

#207649

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT006

Plasmid

#225157

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT005

Plasmid

#225156

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge