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Plasmid#32448PurposeMammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertB-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…PromoterCMVAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
LAMP-CeNL_pcDNA3
Plasmid#89514PurposeCyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorInsertCeNL
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Carl-ReNL-KDEL_pcDNA3
Plasmid#89535PurposeRed color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertReNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Carl-YeNL-KDEL_pcDNA3
Plasmid#89516PurposeYellow color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertYeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Carl-CeNL-KDEL_pcDNA3
Plasmid#89509PurposeCyan color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertCeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAMP-OeNL_pcDNA3
Plasmid#89525PurposeOrange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorInsertOeNL
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
UseTagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ
Plasmid#153036PurposeFor CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK18sB
Plasmid#177838PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA_PDS
Plasmid#46966PurposeExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoterDepositorInsertAtU6p::sgRNA_PDS
UseCRISPR; Plant expressionTagsExpressionMutationPromoterAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB227
Plasmid#68618PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLC-237
Plasmid#73191PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.DepositorInsertParaB-relE
UseTagsExpressionBacterialMutationPromoterParaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-YKT6
Plasmid#116946PurposeExpressing GFP-YKT6 for mammalian cellsDepositorInsertSynaptobrevin homolog YKT6 (YKT6 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB101
Plasmid#68555PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only