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Plasmid#222994PurposeMammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II WT
Plasmid#35175DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hFrataxin-HA
Plasmid#31895DepositorInsertFrataxin (FXN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
negMaLionG
Plasmid#113907Purposenegative control for Green ATP sensorDepositorInsertnegMaLionG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-FLEX-eNpHR-EYFP
Plasmid#89876PurposeTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoterDepositorInserteNpHR=EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterTetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-terminator (SL695)
Plasmid#133961PurposepUC19 plasmid with U6 promoter followed by a poly-T termination signalDepositorInsertU6 promoter
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterSP01Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+LuxG13
Plasmid#26161DepositorInsertBacterial luciferase operon + G13 promoter
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 WT
Plasmid#12605DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.2
Plasmid#32446PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)DepositorArticleInsertG-GECO1.2
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PPP2R1A_WT
Plasmid#81760PurposeGateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.DepositorInsertPPP2R1A (PPP2R1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP11-His3MX
Plasmid#91951PurposeUniversal C-terminal GFP11 taggingDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
UseTagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-pBAD
Plasmid#54572PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 456DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, T7 tag, and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits