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pTH748-CEN-RLuc/min8maxCFLuc

Plasmid

#38214

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first eight codons of the original FLuc gene …

Promoter

ADH1 and TDH3 (=GDP)

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTH753-CEN-RLuc/min346maxCFLuc

Plasmid

#38219

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 346 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH747-CEN-RLuc/min4maxCFLuc

Plasmid

#38213

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first four codons of the original FLuc gene w…

Promoter

ADH1 and TDH3 (=GDP)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-Luc-KLF4-K5S-Mt344B

Plasmid

#34602

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206A

Plasmid

#34599

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-Tau-cYFP

Plasmid

#92205

Purpose

Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(156-239)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pdCas9-DNMT3A-EGFP

Plasmid

#71666

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-tdTomato

Plasmid

#153019

Purpose

Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

P2A-tdTomato

Expression

Mammalian

Mutation

C128S/D156A/T159C

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Use

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MKK1K97A

Plasmid

#107974

Purpose

expression of a dominant negative MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 K97A (MAP2K1 Human)

Use

Tags

Expression

Mammalian

Mutation

dominant negative; see Depositor Comments below

Promoter

CMV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Human IFN-gamma promoter/enhancer

Plasmid

#17598

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter with 1st intron enhancer (IFNG Human)

Use

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Tags

Expression

Mutation

PuroR: Changed Methionin 1 to Glycine.

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-MB-Dlk1

Plasmid

#108930

Purpose

GFP-tagged vector expressing membrane-bound Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

lacks coding sequence for the juxtamembrane motif…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

Plasmid

#154089

Purpose

Improved version to express RUNX1, P2A, ERG in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-KLF4-P2A-mCherry

Plasmid

#203855

Purpose

Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

KLF4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS CoV-2 RBD (bio-His)

Plasmid

#166857

Purpose

Human expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.

Depositor

Evelien Bunnik

Article

Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

Insert

SARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)

Use

Tags

6xHis and Biotinylation site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

Plasmid

#172640

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESh-GFPII-S-Dlk1

Plasmid

#108931

Purpose

GFP-tagged vector expressing secreted isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

contains sequences from full length Dlk1A up to t…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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