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pLEX307_PDHA2S293A

Plasmid

#115197

Purpose

Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

p.S293A

Promoter

EF1alpha

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR3C1-P2A-mCherry

Plasmid

#203854

Purpose

Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTH734-2µ-RLuc/stopCFLuc

Plasmid

#40609

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase (nonsense mutant)
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTN6kwh

Plasmid

#44724

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND3

Plasmid

#172623

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND2

Plasmid

#172629

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM3

Plasmid

#72210

Purpose

Mammalian expression of aa 227-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-226

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM2

Plasmid

#72209

Purpose

Mammalian expression of aa 1-517 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 518-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CCND1(T286A)

Plasmid

#172631

Purpose

Expresses untagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHE Short

Plasmid

#137697

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHG Short

Plasmid

#137699

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Duet-ECHA(37-763)-FLAG (no His-tag)

Plasmid

#96887

Purpose

expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Use

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36

Promoter

T7

Available since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTH752-CEN-RLuc/min103maxCFLuc

Plasmid

#38218

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 103 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-HA, Pbl-A PH+DH domains

Plasmid

#69793

Purpose

Pebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble DH +PH domains (pbl Fly)

Use

P element-based puast vector for gal4-regulated e…

Tags

HA tag

Expression

Insect

Mutation

Pbl-A (NP_729306.1) amino acids 385-647.

Promoter

hsp70 promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only