-
Plasmid#134125PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255 and K183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.13
Plasmid#133534PurposesfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW072M
Plasmid#115960PurposeDestination VectorDepositorInsertLvl2 (nsP2 Q739L)
UseSynthetic BiologyTagsExpressionMutationnsP2 Q739LPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW065
Plasmid#115952PurposeDestination VectorDepositorInsertLvl0 3'UTR Dest
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 no-stop
Plasmid#123211PurposeGateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW103
Plasmid#115946PurposeORF insert for replicon MoCloDepositorInsertLvl0 L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW086M
Plasmid#115934PurposeORF insert for replicon MoCloDepositorInsertLvl0 mKate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW087
Plasmid#115935PurposeORF insert for replicon MoCloDepositorInsertLvl0 EBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW120
Plasmid#115947PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR SapI/BsaI Mut
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW085
Plasmid#115933PurposeORF insert for replicon MoCloDepositorInsertLvl0 mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.S61P
Plasmid#105048PurposeOverexpression of APOBEC3C S61P mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationserine 61 to prolinePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.D99E
Plasmid#105049PurposeOverexpression of APOBEC3C D99E mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationAspartate 99 to glutamatePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.DS-EP
Plasmid#105050PurposeOverexpression of APOBEC3C DS-EP mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationS61P, D99EPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C.C97S
Plasmid#105051PurposeOverexpression of APOBEC3C C97S mutant in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationcysteine 97 to serinePromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
NDUFAB1 C3.3 gRNA
Plasmid#90791Purpose3rd generation lentiviral gRNA plasmid targeting human NDUFAB1DepositorInsertNDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
NDUFAB1 C4.3 gRNA
Plasmid#90793Purpose3rd generation lentiviral gRNA plasmid targeting human NDUFAB1DepositorInsertNDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN3
Plasmid#51448PurposeExpresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N3 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150Q, K153Q, and R154QPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN3
Plasmid#51449PurposeExpresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N3 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150Q, K153Q, and R154QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mc2r-mod
Plasmid#48173Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame elementDepositorInsert5" UTR of MC2R (MC2R Human)
UseLuciferaseTagsExpressionMutationA/G MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Kdr-mod
Plasmid#48177Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame elementDepositorInsert5" UTR of 2KDR (MC2R Human)
UseLuciferaseTagsExpressionMutationA/G MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only