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Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-TagRFP-PuroR
Plasmid#80971PurposeCRISPaint gene taggingDepositorInsertTagRFP
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin
Plasmid#118710PurposeLentiviral TET-ON inducible GFP-ProgerinDepositorInsertGFP-Progerin (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationPromoterCMV TRE3G (TET-ON)Available sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-ARID1A
Plasmid#39311DepositorInsertARID1A (ARID1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shTEAD1/3/4-1
Plasmid#193672PurposeConstitutive lentiviral expression of TEAD shRNADepositorInsertTEAD
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFR D770-N771 insNPG
Plasmid#11016PurposeRetroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cellsDepositorInsertEGFR D770-N771 insNPG (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationD770-N771 insNPGPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-RGVG-M6
Plasmid#70172PurposeExpresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNADepositorInsertRGVG-M6 T7 RNA polymerase
UseTagsHisExpressionBacterialMutationPromoterT5/LacAvailable sinceDec. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEVS104
Plasmid#207393PurposeConjugal helper plasmidDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJC005.em
Plasmid#182738PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KMT2A_MLLT10
Plasmid#205843PurposeExpress mEGFP-tagged fusion protein, KMT2A_MLLT10 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mGM-CSF
Plasmid#74465Purposeexpression of mGM-CSF in mammalian cellsDepositorInsertgranulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3520
Plasmid#144996PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q)
Plasmid#170125PurposeLentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signalDepositorInsertMCP-ADAR2-DD(E488Q)-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_219
Plasmid#164559PurposeLentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR and Lentiviral; Dual sgrnaTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
F2R-Tango
Plasmid#66276PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertF2R (F2R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.22_mCherry-MS2x8
Plasmid#205537PurposeExpress mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-5'-ENGRAM
Plasmid#179157PurposepiggyBac plasmid for 5' ENGRAM recorderDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterminPAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F1
Plasmid#24225PurposeMammalian expression of human E2F1 with HA tagDepositorInsertE2F1 (E2F1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP
Plasmid#176280PurposeViral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBac-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTH
Plasmid#131636PurposeExpresses APOBEC1 fused to the YTH domainDepositorInsertAPOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxSTOPlox-LynEGFP
Plasmid#133922PurposeReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombinationDepositorInsertLynEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF123
Plasmid#135921PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lsg-GBA-1 + 2
Plasmid#199276Purposenicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3DepositorInsertGBA nick
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only