Showing: 4021 - 4040 of 18075 results
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Plasmid#52693PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6AvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-3xHA-LIN-41
Plasmid#52717Purposeretroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-EGR1
Plasmid#52724Purposeretroviral expression of human EGR1DepositorInsertEGR1 (EGR1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-HMGA2
Plasmid#52727Purposeretroviral expression of human HMGA2DepositorInsertHMGA2 (HMGA2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-KRAS
Plasmid#52729Purposeretroviral expression of human KRASDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET11a-link-NGFP
Plasmid#52732PurposeIPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-NGFP
UseTagsHis tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-link-CGFP
Plasmid#52733PurposeL-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NGFP
Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
UseTagsHis tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 Rat, with human c-terminus)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-EGFP-Kir4.1
Plasmid#52874PurposeExpress EGFP-Kir4.1 fusion protein in astrocytesDepositorInsertKir4.1 (Kcnj10 Rat)
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos-FLAG MDA5 MI
Plasmid#52875PurposeExpresses MDA5 with a mutation to motif I in mammalian cellsDepositorInsertIFIH1 (IFIH1 Human)
UseTagsFLAGExpressionMammalianMutationK335A, E658KPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos-FLAG MDA5 MIII
Plasmid#52876PurposeExpresses MDA5 with a mutation to motif III in mammalian cellsDepositorInsertIFIH1 (IFIH1 Human)
UseTagsFLAGExpressionMammalianMutationT488A S490APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos-FLAG RIG-I MIII
Plasmid#52877PurposeExpresses RIG-I with a mutation to motif III in mammalian cellsDepositorInsertDDX58 (RIGI Human)
UseTagsFLAGExpressionMammalianMutationT409A, S411APromoterAvailabilityAcademic Institutions and Nonprofits only -
pBABE lucIL6
Plasmid#52884Purposeexpresses a firefly luciferase mRNA fused to the 3' UTR of IL6DepositorInsertfirefly luciferase fused to IL6 3'UTR (IL6 Human, firefly)
UseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
Plasmid#52889Purpose"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expressionDepositorInsertGal4 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-MCS
Plasmid#52908PurposeAedes aegypti polyubiquitin promoter followed by MCSDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAe aegptyi polyubiquitinAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RPS6 (pDD1902)
Plasmid#52913Purposecontains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.DepositorInsertHAS6 (RPS6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLKO-shp38
Plasmid#52920Purposeexpresses a hairpin specific for human p38MAPKDepositorInsertshRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 4021 - 4040 of 18075 results