-
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire1_E2F1RE
Plasmid#112248Purposeexpression of GFP and Luciferase under the control of E2F1 responsive elementDepositorInsertE2F1RE (responsive element)
UseLentiviralTagscopGFP and luciferaseExpressionMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDACR3882 [rab-3p::HYlight::let-858utr]
Plasmid#207666PurposeExpresses worm-optimized HYlight in C. elegans neuronsDepositorInsertHYlight Biosensor
UseTagsExpressionWormMutationPromoterrab-3Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDACR3883 [rab-3p::HYlight-RA::let-858utr]
Plasmid#207667PurposeExpresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neuronsDepositorInsertHYlight-RA [T152E]
UseTagsExpressionWormMutationPromoterrab-3Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-NCAM-1
Plasmid#205190PurposeThis vector encodes of a synCAM (NCAM1) and GFPDepositorInsertsynCAM NCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorInsertRNASEH1 (nuclear isoform) (RNASEH1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorInsertH2B tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-BE4max
Plasmid#183679PurposeExpresses eNme2-C-BE4 in mammalian cellsDepositorInsertrAPOBEC1-neNme2-C-2xUGI
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG153 FKBP-CFAST11
Plasmid#130813PurposeExpresses FKBP-CFAST11 in mammalian cellsDepositorInsertFKBP-CFAST11
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 ORF3a C-V5-tag
Plasmid#179988PurposeMammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 ORF3a
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag-JunB
Plasmid#29687DepositorInsertjun B oncogene (JUNB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-Stim1
Plasmid#186620PurposeThird generation lentiviral vector expressing Stim1-wt fused to mCherryDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317 TEAD2
Plasmid#193686PurposeConstitutive lentiviral expression of TEAD2DepositorInsertTEAD2 (TEAD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM82
Plasmid#133648PurposeIntegration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mCherry (NAB2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7
Plasmid#180315Purposemammalian expression of human SEPT7 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phi3-GCaMP6f
Plasmid#106298PurposeCMV driven GFP based calcium (Ca2+) reporterDepositorInsertGCaMP6f
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV PRDM16
Plasmid#15504DepositorInsertPRDM16 (isoform 4) (Prdm16 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorInsertKDR (KDR Human)
UseTagsLabeled with mCherry on the C-Terminus via a 3 am…ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL4A-FLAG
Plasmid#15821DepositorInsertCullin4A (CUL4A Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N440PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4
Plasmid#27002DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-beta-HA-BRCA2
Plasmid#133553PurposeMammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORFDepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorInsertNdi-1 (NDI1 Budding Yeast)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
IgK-IFN-luc
Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorInsertIgK-IFN (IFNB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A8_STOP
Plasmid#161155PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A8 (SLC22A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNW-sfGFP(SPS6)
Plasmid#133814PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPptaAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGCN2-FLAG-IP
Plasmid#101794PurposeRetroviral vector encoding C-terminally FLAG tagged mouse GCN2DepositorInsertGcn2 (Eif2ak4 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Golgi-7
Plasmid#55052PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorInsertGolgi (B4GALT1 Human)
UseTagsmCherryExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHuji (CD63 Human, synthetic)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GRK2-K220R
Plasmid#35403DepositorInsertGRK2 (GRK2 Bovine)
UseTagsExpressionMammalianMutationK220RPromoterCMVAvailable sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only