Addgene

Showing: 3981 - 4000 of 12906 results

Results per page:

Sort results by:

p414GPD-3xFLAG-Nop4 dE8

Plasmid

#169264

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E8 (NOP4 Budding Yeast)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 8

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTUB1:OsTIR1-3FLAG, SAG1:CAT

Plasmid

#87258

Purpose

TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

TIR1
CAT

Use

Toxoplasma expression

Tags

3FLAG

Expression

Mutation

Codon optimized for Toxoplasma gondii expression

Promoter

TgSAG1 361 bp and TgTUB1 2717 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

Plasmid

#52504

Purpose

mammalian expression vector of BRCA1 wildtype siRNA resistant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

siRNA resistant sequence: AGTATAATC

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLL8 lenti-D10Acas9-3xflag-blast

Plasmid

#112134

Purpose

lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

D10Acas9-3xflag-blast

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A mutant in Cas9

Promoter

EF1a

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Article

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1 alpha

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-EGFP-FLAG-cGAS

Plasmid

#127661

Purpose

Over-express EGFP-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-Flag

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Use

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag CKMT1A

Plasmid

#20459

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT1A (CKMT1A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-GFP-EphrinB3-Flag(A227Y)

Plasmid

#155013

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

A227Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Flag-YAP-5SA/S94A

Plasmid

#33103

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S61A, S94A, S109A, S127A, S164A, S381A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-CDK8-WT-IRES-mCherry

Plasmid

#68856

Purpose

Expresses Human CDK8 Wild-Type

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-CDK19-WT-IRES-ZsGreen

Plasmid

#68858

Purpose

Expresses Human CDK19 Wild-Type

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge