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Addgene
Showing: 3981 - 4000 of 4729 results
  1. pGL2-hOct4 DE-SV40-Luc

    Plasmid
    #26279
    Purpose
    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
    Depositor
    Insert
    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments below
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
  2. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid
    #176244
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Ribosomal subunit
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pGECPL.BC10.MG

    Plasmid
    #223822
    Purpose
    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.
    Depositor
    Insert
    Ef1a-Driven Cre-P2A-FLuc
    Use
    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pGL3-pSMANTIS_SMAD-FOS_Mutant

    Plasmid
    #194188
    Purpose
    Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated SMAD/FOS Site, Region 3-15nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid
    #194189
    Purpose
    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated STAT1/2 site, Region 653-673nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3981 - 4000 of 4729 results