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RCAS GH6 (CC#152)
Plasmid#15137DepositorInsertGH6 (HMX1 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS SOHo1 (CC#99)
Plasmid#15132DepositorInsertSOHo1 (SOHO-1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
C3AR1-Tango
Plasmid#66231PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertC3AR1 (C3AR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHCMV_FLUAV_HA
Plasmid#207273PurposeMammalian expression of Influenza A virus HA proteinDepositorInsertFLUAV_HA (HA Influenza A virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVlux-cp157Venus
Plasmid#197584PurposeBRET lux luciferase with cp157Venus for mammalian expressionDepositorInserthuman codon-optimized Lux-cp157Venus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-MYOD1-shOct4
Plasmid#182309PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationshOct4PromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XFLAG-EGFP-OMP25
Plasmid#83354PurposeFor tagging mitochondria with Sigma FLAG epitopesDepositorInsert3XFLAG-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF-miRFP709-hCdt(1/100)
Plasmid#80007PurposehCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)DepositorInserthCdt1(1/100) (CDT1 Human)
UseLentiviralTagsmiRFP709ExpressionMutationPromoterEF-1 alphaAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM040 [Multiome Perturb-seq sgRNA expression vector]
Plasmid#224898PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experimentsDepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-Puro
Plasmid#207743PurposeExpression of the Cre recombinase linked to a puro selectable markerDepositorArticleInsertCre-2A-Puro
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXL001-TQM IL13 CAR
Plasmid#154054PurposeLentiviral expression of TQM IL13 CAR for CAR-T therapyDepositorInsertTQM IL13 CAR
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRII_TOPO_hNEAT1
Plasmid#61518Purposehuman NEAT1_v1 short isoform 3.7kb insert in general cloning vectorDepositorInsertNEAT1 (NEAT1 Human)
UseGeneral cloning vectorTagsExpressionMutation*see comment belowPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas ServiceAAV PHP.eBInsertGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx7
Plasmid#45921DepositorInsertsyntaxin 7 (STX7 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1
Plasmid#118580Purposeto overexpress human MALAT1 in mammalian cellsDepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Delta(Pro-MP)ADAM17-HA
Plasmid#65221PurposeExpression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cellsDepositorInsertDelta(Pro-MP)ADAM17 (ADAM17 Human)
UseTagsHAExpressionMammalianMutationdeleted AA 18-473PromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Flag-humanRBM47
Plasmid#134607PurposePlasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.DepositorArticleInsertFlag-humanRBM47 (RBM47 Human)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL3
Plasmid#149518PurposeExpress APEX2-Galectin-3 in mammalian cellsDepositorInsertLGALS3 (LGALS3 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloExpressionMutationPromoterSFFVAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only
