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pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overespression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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PDHA1-N-10

Plasmid

#58195

Purpose

Localization: Mitochondria, Excitation: N/A, Emission: N/A

Depositor

Michael Davidson

Insert

PDHA1 (PDHA1 Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
R777-E231 Hs.RASA3

Plasmid

#70515

Purpose

Gateway ORF clone of human RASA3 [NM_007368.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RASA3 (RASA3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 Peroxisome visual barcode

Plasmid

#158668

Purpose

tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-NTERM-GFP

Plasmid

#185764

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

William Sellers, Alessandra Ianari

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

C-terminal V5-tagged BirA

Use

Tags

V5, His

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dio-GFP

Plasmid

#198279

Purpose

Cre reporter for mammalian cells; double-floxxed-EGFP

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Dio-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-GATA4

Plasmid

#192896

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

GATA4 (GATA4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Codon-optimized

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

William Sellers, Alessandra Ianari

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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EB3-mNeonGreen

Plasmid

#98881

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

EB3

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPSU40

Plasmid

#173831

Purpose

Expresses 40 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPACGST

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJB158

Plasmid

#202601

Purpose

RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

Depositor

Athanasios Typas

Article

Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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