Addgene

Showing: 381 - 400 of 3250 results

Results per page:

Sort results by:

pYX233

Plasmid

#163762

Purpose

Empty vector for Gal-inducible yeast expression

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Type

Empty backbone

Use

Tags

Hemagglutinin (HA) tag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHDY-Prha-mVenus_rhaS

Plasmid

#137662

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

Prha:mVenus

Use

Tags

Expression

Bacterial

Mutation

rhaS (Arg218Leu)

Promoter

Prha

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU1-A-SP44-mVenus-I

Plasmid

#163763

Purpose

Expresses Streptomyces codon-optimised mVenus-I

Depositor

Simon Moore, Paul Freemont

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mVenus-I

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

SP44

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-RhebL1

Plasmid

#133766

Purpose

Expresses GFP-tagged RhebL1 in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

RhebL1 (RHEBL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

Plasmid

#110376

Purpose

Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Tags

FLAG and tGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-Fascin-C-10

Plasmid

#57376

Purpose

Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Fascin (FSCN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aa3.8

Plasmid

#136376

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG-D-mSpyCatcher-His6-E

Plasmid

#87376

Purpose

An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

mSpyCatcher

Use

Synthetic Biology

Tags

His6 tag

Expression

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
2xStrep-ORF34 pCDNA4

Plasmid

#120376

Purpose

Expresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF34 (ORF34 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

2xStrep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0006L

Plasmid

#178376

Purpose

Expresses KSHV K2 ORF tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K2

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK429

Plasmid

#155376

Purpose

PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEI071

Plasmid

#105376

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor CAB3 (At1g29910; 1000bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-Kan_TH16_AVR-Pib_68

Plasmid

#121376

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH16_AVR-Pib_68

Use

Golden gate

Tags

Expression

Mutation

Magnaporthe oryzae

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EcPhnD90.L1∆∆AD.L297RL301R.pRSET

Plasmid

#33376

Purpose

a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

Depositor

Loren Looger

Article

Alicea et al J Mol Biol. 2011 Oct 12.

Insert

phnD based Phosphonate Biosensor (phnD E. coli)

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted in place of…

Promoter

T7

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB560-NADK2

Plasmid

#203760

Purpose

For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

NAD KINASE2

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RVD-GT9

Plasmid

#173376

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NN-NG dimer RVD at Position9

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Use

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge