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Plasmid#176626PurposeTF A self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176627PurposeTF B self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TetON-dual-SunTag-Hygro
Plasmid#121119PurposeEndogenous gene activation with the SunTag CRISPR activation systemDepositorInsertdCas9-GCN4x10_scFv-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterTet-ONAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176628PurposeTF A self-activation cassette in MultiFate-2.3DepositorInsert14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176624PurposeTF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3DepositorInsertTRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Plasmid#176629PurposeTF C self-activation cassette in MultiFate-3DepositorInsert14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-MCS-NLS-3XFLAG-V5
Plasmid#196383PurposeEmpty backbone vector to insert cDNA for strong expression in mammalian cellsDepositorTypeEmpty backboneUseTags3xFLAG and V5ExpressionBacterial and MammalianMutationPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.18_PB-SDC4-PGK-Blast
Plasmid#205563PurposeExpress human SDC4DepositorInsertSDC4 (SDC4 Human)
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mNeonGreen
Plasmid#175508PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176625PurposeTF A self-activation cassette in MultiFate-2.1DepositorInsertTRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast
Plasmid#205549PurposeExpress EPN24-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-EGFP
Plasmid#133400PurposePiggybac vector for constitutive expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1153_pPBCAG-MerCreMer-IN
Plasmid#124183PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast
Plasmid#205546PurposeExpress EPN11-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS
Plasmid#140011PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Antares2
Plasmid#120868PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertpcDNA3-Antares2
UseTagsExpressionMammalianMutationAntares2 ampliconPromoterCAG PromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:
Plasmid#179585PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNLS-NG-T2AExpressionMutationPromoterCAGpAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI
Plasmid#133363PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MGT1-d2EGFP_CMV-mCherry
Plasmid#213763PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-PNGT3-d2EGFP_CMV-mCherry
Plasmid#213765PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertPNGT#3-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
KA1158_pPBCAG-MerCreMer-IZ
Plasmid#124184PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Zeo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_GFP 150TAG
Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1055J
Plasmid#200251PurposepBac-U6-gRNA(Intersex)-3xp3-tdTomatoDepositorInsert2 gRNAs targeting Intersex gene
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
1154B_gFLE_pB_VTK_ActG
Plasmid#187238PurposeAn. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fleDepositorInsertFemaleless
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast
Plasmid#205542PurposeExpress EPN1-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast
Plasmid#205541PurposeExpress EPN1-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast
Plasmid#205545PurposeExpress EPN11-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_GFP 150TAG
Plasmid#140017PurposePlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT sfGFP 150TAG
Plasmid#154771Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
Plasmid#233413PurposeLIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as outputDepositorInsertLIDARSensor(mCherry->Casp9-2A-GFP)-hygro
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-DEST (JDW 1205)
Plasmid#229821PurposeA PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only