Addgene

We narrowed to 1,445 results for: Mbp

Showing: 571 - 600 of 1445 results

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T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Use

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAcGP67B MBP-tagged human Norrin ( 31–133)

Plasmid

#132625

Purpose

expresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

Insert

MBP-Norrin ( 31–133)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (830-839)

Plasmid

#188091

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (830-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_L58R

Plasmid

#101780

Purpose

Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

L58R mutation

Promoter

T5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28 MBP-TEV-Rat BCKDK-His

Plasmid

#232119

Purpose

For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

BCKDK (Bckdk Rat)

Use

Tags

6 x His, Maltose-Binding Protein (MBP), and Tev S…

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X(D354V)-monoGFP-His

Plasmid

#219986

Purpose

DDX3X(D354V) protein purification

Depositor

Simon Alberti

Insert

DDX3X(D354V) (DDX3X Human)

Use

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Mutation

D354V

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X(G325E)-monoGFP-His

Plasmid

#219987

Purpose

DDX3X(G325E) protein purification

Depositor

Simon Alberti

Insert

DDX3X(G325E) (DDX3X Human)

Use

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Mutation

G325E

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB-2STREP-HsHelz_1051-1942-GSSG-MBP_AG

Plasmid

#148795

Purpose

Mammalian Expression of HsHelz_1051-1942-GSSG

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1942-GSSG (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-T411EL415E_AG

Plasmid

#148778

Purpose

Mammalian Expression of HsNot4_275-575-T411EL415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411EL415E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-F405D_AG

Plasmid

#148773

Purpose

Mammalian Expression of HsNot4_275-575-F405D

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F405D (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-F408A_AG

Plasmid

#148774

Purpose

Mammalian Expression of HsNot4_275-575-F408A

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F408A (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-FFTLtoDAEE_AG

Plasmid

#148775

Purpose

Mammalian Expression of HsNot4_275-575-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-FFTLtoDAEE (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-L415E_AG

Plasmid

#148776

Purpose

Mammalian Expression of HsNot4_275-575-L415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-L415E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-T411E_AG

Plasmid

#148777

Purpose

Mammalian Expression of HsNot4_275-575-T411E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del133-155-V5-SBP-MBP_V

Plasmid

#147763

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del267-809-V5-SBP-MBP_V

Plasmid

#147764

Purpose

Mammalian Expression of HsSmg5-del267-809

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del267-809 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-del810-846-V5-SBP-MBP_V

Plasmid

#147765

Purpose

Mammalian Expression of HsSmg5-del810-846

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del810-846 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-del847-1017-V5-SBP-MBP_V

Plasmid

#147766

Purpose

Mammalian Expression of HsSmg5-del847-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del847-1017 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S

Plasmid

#147471

Purpose

Mammalian Expression of HsSmg5-Q123E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg5-Q123E-siRNAres (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

three non silent mutation A254G (K85R), T980C (L3…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg6_577-816-siRNAres-V5-SBP-MBP_I

Plasmid

#146570

Purpose

Mammalian Expression of HsSmg6_577-816-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_577-816-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG

Plasmid

#148841

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (801-839)

Plasmid

#188089

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (723-839)

Plasmid

#188088

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (723-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-Foldon-ATG9 (828-839)

Plasmid

#188090

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (828-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4-del190-274_AG

Plasmid

#148782

Purpose

Mammalian Expression of HsNot4-del190-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del190-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 571 - 600 of 1445 results

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