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AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

Plasmid

#120293

Purpose

AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

N-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

SV40-NLS and split-intein

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MF3958 Heavy chain-SpyTag003

Plasmid

#216303

Purpose

Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

MF3958 Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-SpyTag003

Plasmid

#216293

Purpose

Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-39S Heavy chain-SpyTag003

Plasmid

#216296

Purpose

Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Omicron XBB.1.5

Plasmid

#214724

Purpose

Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Use

Tags

SpyTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115G)-Talin rod-mCherry

Plasmid

#210026

Purpose

Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115G mutation to modify SpyCatcher003 …

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115A)-Talin rod-mCherry

Plasmid

#210025

Purpose

Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115A mutation to modify SpyCatcher003 …

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003(K31TAG)-Talin rod-mScarletI

Plasmid

#210022

Purpose

Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI

Use

Tags

C-terminal mScarletI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003(K31TAG)

Plasmid

#210023

Purpose

Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)

Use

Tags

Expression

Mammalian

Mutation

SpyCatcher003 with K31TAG (Amber stop codon) muta…

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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