Addgene

We narrowed to 274 results for: vasa

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SpVasa-K332N-GFP

Plasmid

#185592

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-K332N

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

K332N

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S636K-GFP

Plasmid

#185589

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S636K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

S636K

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Tags

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster vasa

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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F15-pAc5.1 EGFP Vasa

Plasmid

#206483

Purpose

Vasa expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-VASA-tdTomato-pNeo

Plasmid

#141030

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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F17-pAc5.1 EGFP Vasa 1-200

Plasmid

#206484

Purpose

vasa 1-200 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa 1-200

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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F16-pAc5.1 EGFP Vasa closed

Plasmid

#206487

Purpose

Vasa (closed conformation) expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

vasa E400Q

Use

Tags

EGFP

Expression

Insect

Mutation

E400Q

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165887

Purpose

G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Vasa open (K295N) (RR228)

Plasmid

#206435

Purpose

Vasa expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa (vas Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

K282N

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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F19-pAc5.1 EGFP Vasa 463-661

Plasmid

#206486

Purpose

Vasa 463-661 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa 463-661

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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F18-pAc5.1 EGFP Vasa 200-462

Plasmid

#206485

Purpose

Vasa 200-642 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa 200-462

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165888

Purpose

Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52546

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52544

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52543

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52547

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge