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pCDNA3.0_mitoLAMA-G97 IRES EGFP

Plasmid

#130707

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

Plasmid

#130708

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

Plasmid

#130709

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCB268

Plasmid

#53363

Purpose

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

Depositor

Alexander Varshavsky

Article

Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

Inserts

DHFR (Dhfr Mouse)
Ub
Aβ42 (APP Human)

Use

Tags

13xMyc, Flag, and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
N-SF-TAP-DEST-TLK2-D592V

Plasmid

#218835

Purpose

Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

Depositor

Travis Stracker

Insert

TLK2 Q592V (kinase dead) (TLK2 Human)

Use

Tags

FLAG-STREP

Expression

Mammalian

Mutation

Q592V

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC2

Plasmid

#22975

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC2 (CRTC2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pMAH-ER-bapaUGAc

Plasmid

#201674

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

ER-bapaUGAc

Use

Tags

ER leading sequence and ER retention sequence

Expression

Mammalian

Mutation

Promoter

CMV SP6

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-Golgi-bapaUGAc

Plasmid

#201675

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

Golgi-bapaUGAc

Use

Tags

Golgi leading sequence

Expression

Mammalian

Mutation

Promoter

CMV SP6

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Use

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-tagRFP

Plasmid

#114039

Purpose

Kinetochore localised FRB tagged with FLAG and tagRFP

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Use

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Stat3 pcDNA3

Plasmid

#8706

Purpose

See Depositor Comments below for variant information.

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

* see comments

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-634)

Plasmid

#25718

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-634) (CRTC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3_ReBphP-PCM_IRES_sfGFP

Plasmid

#166917

Purpose

Express ReBphP-PCM and sfGFP

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

ReBphP-PCM
sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Barkor (FL)

Plasmid

#24394

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
N-TAP A2A in pcDNA3

Plasmid

#67850

Purpose

mammalian expression of A2A receptor with N terminal TAP tag

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
N-TAP A2A-YFP pcDNA3

Plasmid

#67852

Purpose

mammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusion

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only