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Showing: 21 - 35 of 35 results

  1. pUltraI-Tet3.0[TAA]

    Plasmid
    #164580
    Purpose
    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L170G, N311G, C313A
    Promoter
    ProK and TacI
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    164580-plasmid-map-sequence-id-392917

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  2. pUltra-Plus-H2B-mTagBFP2

    Plasmid
    #161790
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Bicistronic
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC and UbC promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161790-plasmid-map-sequence-id-316820

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  4. pUltra-Plus-H2B-EGFP

    Plasmid
    #161776
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-EGFP from Addgene Plasmid #26790
    Use
    Lentiviral; Bicistronic
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    UbC and UbC promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161776-plasmid-map-sequence-id-316824

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  5. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

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  6. pUltra-Smurf-luc-RTTA3

    Plasmid
    #59438
    Purpose
    silence doxycyclin inducible pUltra-dox (also expresses luciferase)
    Depositor
    Yildirim Dogan, Kitai Kim
    Inserts
    luciferase
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59438-plasmid-map-sequence-id-92494

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  8. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

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  9. Ultra-Exon1Q23-Myc-A

    Plasmid
    #110486
    Purpose
    Lentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Short 3'UTR wild type huntingtin exon1 fragm…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110486-plasmid-map-sequence-id-212584

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  10. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  12. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  13. pHOT-P2Y2R

    Plasmid
    #159108
    Purpose
    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson
    Depositor
    Fabio Mammano
    Article
    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc
    Insert
    P2Y2R
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    UBC
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159108-plasmid-map-sequence-id-318664

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Showing: 21 - 35 of 35 results