Addgene

We narrowed to 1,576 results for: nif

Showing: 21 - 40 of 1576 results

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CoA-Snifit(G41)

Plasmid

#197945

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CoA-Snifit(V97T)

Plasmid

#197947

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41)

Plasmid

#197939

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Bacterial

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(V97T)

Plasmid

#197941

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Bacterial

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_CoA-Snifit(V97T)

Plasmid

#197944

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(G41)

Plasmid

#197942

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SNIFP-P2A-GFP

Plasmid

#197157

Purpose

Expresses the protein of SNIFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

SNIFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_COX8-CoA-Snifit(G41S)

Plasmid

#197946

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

Plasmid

#197943

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P shNFS1_1

Plasmid

#102963

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_1 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_2

Plasmid

#102964

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_2 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_4

Plasmid

#102966

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_4 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_3

Plasmid

#102965

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_3 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe hygro NFS1

Plasmid

#102977

Purpose

Expression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 (NFS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Silent mutations to eliminate shNFS1_1 binding si…

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR sgNFS1

Plasmid

#102979

Purpose

Cutting NFS1 genomic locus

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

sgNFS1 (NFS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Promoter

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
pET-MBP-Geo_st

Plasmid

#87700

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

Depositor

Jennifer Doudna

Insert

GeoCas9

Use

Tags

10xHis-MBP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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