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Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGD425
Plasmid#132381Purposecontains the sequence of mTurquoise2 for C-term fusions in the pGGD000 backbone (GreenGate)DepositorInsertlinker-mTurquoise2
UseGreengate entry plasmidTagsExpressionMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-K-GECO1
Plasmid#191469PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertK-GECO1
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-R-GECO1
Plasmid#191471PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertR-GECO1
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-CH-GECO2.1
Plasmid#191470PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent proteinDepositorInsertCH-GECO2.1
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV AURKA-mTurq2
Plasmid#157767PurposeExpression of AuroraA kinase fused to mTurquoise2 under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV ShadowG-AURKA-mTurq2
Plasmid#157768PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV ShadowY-AURKA-mTurq2
Plasmid#157770PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowYExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV ShadowG-AURKA K162M-mTurq2
Plasmid#157769PurposeExpression of AuroraA kinase-dead biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowGExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV ShadowY-AURKA K162M-mTurq2
Plasmid#157771PurposeExpression of AuroraA kinase-dead biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowYExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPO-His-mTurquoise2_T203Y
Plasmid#191456PurposeBacterial isolation of mTurquoise2 with a T203Y mutationDepositorInsertmTurquoise2_T203Y
UseTagsHisExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mTq2-antiFRET-P2A2-linker_noSacI
Plasmid#191472PurposeNegative control for ratiometric comparison between red fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_018
Plasmid#123831PurposeEncodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertP2a-NLS::mTurquoise
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV superYFP-AURKA-mTurq2
Plasmid#157772PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and superYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only