...conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa = 3.1) and mCherry (...pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown) remains fluorescent in acidic organelles...successfully generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and ...Central PMCID: PMC3918502.  14. Chernov, Konstantin G., et al. "Near-infrared fluorescent proteins, biosensors...et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC biology...

...behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry...from N- to C-terminus. For instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid...al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al., 2001, Adjobo-Hermans...et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC biology.... "Receptor-mediated activation of heterotrimeric G-proteins in living cells." Science 291.5512 (2001)...