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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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RT3GEN

Plasmid

#111165

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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RT3GEP

Plasmid

#111164

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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GSX2-P2A-tGFP-DTA

Plasmid

#161750

Purpose

tGFP plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Article

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

turboGFP

Use

CRISPR

Tags

P2A-tGFP

Expression

Mammalian

Mutation

Promoter

endogenous GSX2 promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ FL

Plasmid

#84024

Purpose

Expression of GFP tagged BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLOC-MCOLN1

Plasmid

#131583

Purpose

For expression of human MCOLN1 in mammalian cells, GFP-tagged

Depositor

Sean Morrison

Article

Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

Insert

MCOLN1 (MCOLN1 Human)

Use

Lentiviral

Tags

turboGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSB65 - pL0_tGFP-I (CDS1)

Plasmid

#123182

Purpose

Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites; codon-optimised for plants

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF1, delta ZF2

Plasmid

#84027

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-58; Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF2

Plasmid

#84026

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 35-58; Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF1

Plasmid

#84025

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-34; Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta GLEBS

Plasmid

#84028

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 357−373; Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10comp

Plasmid

#65428

Purpose

Competitor vector for transcriptional mutagenesis assay

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10T

Plasmid

#65427

Purpose

Parent vector for constructing non-replicating lesion-bearing plasmid

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MPRA_minCMV+GFP

Plasmid

#204866

Purpose

Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Insert

minCMV:GFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

minimal CMV

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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